Long Duration Responses Obtained from Internally Perfused Axons
نویسندگان
چکیده
منابع مشابه
Long Duration Responses Obtained from Internally Perfused Axons
Methods Isolated and cleaned squid giant axons were internally perfused by a modification of the method of Adelman and Gilbert (1964) and Adelman and Fok (1964). A chemically driven syringe (Sage Instruments) was directly connected to the axon interior through a small cut in one end by means of a 200,/ diameter glass cannula. The internal axoplasm was eroded away upon continuous internal fluid ...
متن کاملInternally perfused Myxicola giant axons showing long-term survival.
An improved method for internally perfusing the Myxicola giant axon based on removing the axoplasm by dispersing it in KCl-KF salt solutions is described. Proteolytic enzymes are not introduced. With this improved method perfused preparations show long-term stability of their electrical properties and the ability to generate action potentials for many hours. Mean initial values for resting memb...
متن کاملVoltage Clamp Studies on Internally Perfused Axons
Probably the most striking and surprising observation in internally perfused axons is that full-sized action potentials can be obtained in the face of a very low resting potential when the ionic strength is low In all experiments at normal ionic strength known to the author, the explosive all-or-none responsiveness was lost when the internal and/or external monovalent cations were arranged to g...
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Giant axons from the squid Dosidicus gigas were internally perfused with rabbit antiaxoplasm antibodies and their effect upon the action potential and the membrane potential was studied. Necessary requirements for the antibodies to affect these parameters in a consistent manner were: (a) removal of the bulk of axoplasm from the perfused zone, accomplished by initially perfusing with a cysteine-...
متن کاملComparison of Tetrodotoxin and Procaine in Internally Perfused Squid Giant Axons
Squid giant axons were internally perfused with tetrodotoxin and procaine, and excitability and electrical properties were studied by means of current-clamp and sucrose-gap voltage-clamp methods. Internally perfused tetrodotoxin was virtually without effect on the resting potential, the action potential, the early transient membrane ionic current, and the late steady-state membrane ionic curren...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1965
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.48.5.1